id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103055-071a5b0x	Rathbun, LI	PLK1- and PLK4-mediated asymmetric mitotic centrosome size and positioning in the early zebrafish embryo	2020-04-14		.txt	text/plain	3126	156	49	We propose a model in which uniquely large centrosomes direct spindle placement within the disproportionately large zebrafish embryo cells to orchestrate cell divisions during early embryogenesis. This is clearly visualized through the use of a fluorescent microtubule transgenic zebrafish line (EMTB-3xGFP) [7] , where the 16-cell stage embryo contains mitotic spindles oriented perpendicular to the previous division at the 8 In early development, rapid rounds of division result in a stark decrease in cell size during the cleavage stage [8] . Strikingly, mitotic centrosomes in zebrafish embryos were extremely large with g-tubulin organized into a wheel-like structure (246.44±11.93μm 2 at 8-cell stage, Figure 1E ), compared to g-tubulin in C. elegans and zebrafish embryos, cell length and mitotic centrosome area decreased by 30-40% over time. In order to determine the importance of PLK1/4-dependent asymmetric mitotic centrosome size placement in early zebrafish divisions, we raised embryos after injection of 1% DMSO, 1μM BI2536, or 1μM centrinone ( Figure 4F ).	./cache/cord-103055-071a5b0x.txt	./txt/cord-103055-071a5b0x.txt