id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103163-0rreoh4o	Smith, Sydni Caet	Reovirus RNA recombination is sequence directed and generates internally deleted defective genome segments during passage	2020-10-22		.txt	text/plain	8965	456	46	We determined the titers and RNA segment profiles of reovirus (rsT1L and rsT3D I ) and rotavirus (rsSA11) laboratory strains that had been rescued by reverse genetics then serially passaged ten times, each in triplicate lineages, in cultured cells. The two reoviruses accumulated non-canonical RNAs that retain 5′ and 3′ termini and feature one or more large internal deletions, while the rotavirus rarely accumulated such DVGs. Analyses of next-generation RNA-sequencing data sets from purified rsT1L reovirus RNA revealed many junctions, with hot spots for recombination in specific viral gene segments. Taken together, lin1 RNA profiles suggest non-canonical RNA species that differ in length but have identical termini to the parental segments accumulate variably during reovirus and rotavirus serial passage in cultured cells.	./cache/cord-103163-0rreoh4o.txt	./txt/cord-103163-0rreoh4o.txt