id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103174-4m3ajc8a	Okada, Megan	Doxycycline has Distinct Apicoplast-Specific Mechanisms of Antimalarial Activity	2020-10-16		.txt	text/plain	2809	148	49	Doxycycline (DOX) is a key antimalarial drug thought to kill Plasmodium parasites by blocking protein translation in the essential apicoplast organelle. Exogenous iron rescues parasites and apicoplast biogenesis from first-but not second-cycle effects of 10 μM DOX, revealing that first-cycle activity involves a metal-dependent mechanism distinct from the delayed-death mechanism. We observed that IPP shifted the 48-hour EC50 value of DOX from 5 ± 1 to 12 ± 2 µM 100 (average ± SD of 5 independent assays, P = 0.001 by unpaired t-test) ( Figure 2C and Figure 2 -101 figure supplement 1), suggesting that first-cycle growth defects from 5-10 µM DOX reflect an 102 apicoplast-specific mechanism but that DOX concentrations >10 µM cause off-target defects 103 outside this organelle.	./cache/cord-103174-4m3ajc8a.txt	./txt/cord-103174-4m3ajc8a.txt