id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103181-my4n0vye	Valle-Casuso, José Carlos	Replication of Equine arteritis virus is efficiently suppressed by purine and pyrimidine biosynthesis inhibitors	2020-04-10		.txt	text/plain	2171	115	47	Using this assay, we identified three molecules that impair EAV infection in equine cells: the broad-spectrum antiviral and nucleoside analog ribavirin, and two compounds previously described as inhibitors of dihydroorotate dehydrogenase (DHODH), the fourth enzyme of the pyrimidine biosynthesis pathway. We were also interested in exploring the antiviral capacity of two new BSA, IPPA17-04 and GAC50 163 that impair viral replication through inhibition of de novo pyrimidine biosynthesis in host cells. These results show that cell cultures treated with pyrimidine biosynthesis inhibitors did not 175 exhibit cytopathic effects associated to EAV infection, suggesting that EAV replication is also impaired. To verify that ribavirin, IPPA17-A04 and GAC50 actually block EAV replication, viral genome copies 180 were quantified at 48 h.p.i. Culture supernatants of infected ED cells treated or not with the 181 compounds at different concentrations were analyzed by RT-qPCR.	./cache/cord-103181-my4n0vye.txt	./txt/cord-103181-my4n0vye.txt