id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103208-krann2ir	Barber-Axthelm, Isaac M	Coformulation with tattoo ink for immunological assessment of vaccine immunogenicity in the draining lymph node	2020-08-29		.txt	text/plain	2931	146	43	In order to improve the accurate isolation of antigen-exposed lymph nodes during biopsies and necropsies, we developed and validated a method for co-formulating candidate vaccines with tattoo ink, which allows for direct visual identification of vaccine-draining lymph nodes and evaluation of relevant antigen-specific B and T cell responses by flow cytometry. We tested if sampling accuracy, and the characterisation of vaccine-elicited immune responses ex vivo, could be improved using tattoo ink to label vaccine-draining LNs in NHPs. Pigtail macaques (Macaca nemastrina) were immunised in the right quadriceps with SARS-CoV-2 spike (100μg) formulated with monophosphoryl lipid A (MPLA) liposomal adjuvant ( 2 9 ) , and were boosted IM in the right and left quadriceps with SARS-CoV-2 spike (100μg) formulated with MPLA and tattoo ink (1.0%). . Animals were additionally immunised in the right and left deltoids with human immunodeficiency virus-1 (HIV-1) fixed trimeric envelope protein (SOSIP) vaccines (100μg) formulated with MPLA and 1.0% tattoo ink (ink in the right deltoid only), with expected drainage to the axillary LNs (Fig 3A) (	./cache/cord-103208-krann2ir.txt	./txt/cord-103208-krann2ir.txt