id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103320-2rpr7aph	Bhandari, Bikash K.	Solubility-Weighted Index: fast and accurate prediction of protein solubility	2020-03-26		.txt	text/plain	4889	333	50	Results We have discovered that global structural flexibility, which can be modeled by normalised B-factors, accurately predicts the solubility of 12,216 recombinant proteins expressed in Escherichia coli. Protein solubility, at least in part, depends upon extrinsic factors such as ionic strength, temperature and pH, as well as intrinsic factors-the physicochemical properties of the protein sequence and structure, including molecular weight, amino acid composition, hydrophobicity, aromaticity, isoelectric point, structural propensities and the polarity of surface residues (Wilkinson and Harrison 1991; Chiti et al. 2003 ) Among these sets of B-factors, sequence composition scoring using the most recently published set of normalised B-factors produced the highest AUC score ( To improve the prediction accuracy of solubility, we iteratively refined the weights of amino acid residues using the Nelder-Mead optimisation algorithm (Nelder and Mead 1965) . To understand the properties of soluble and insoluble proteins, we determined the enrichment of amino acid residues in the PSI:Biology targets relative to the eSOL sequences (see Methods).	./cache/cord-103320-2rpr7aph.txt	./txt/cord-103320-2rpr7aph.txt