id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103417-2uinislh	Doi, Hideyuki	On-site eDNA detection of species using ultra-rapid mobile PCR	2020-10-01		.txt	text/plain	1507	91	50	Our method reduced the measurement time to 30 min and provided high detectability of aquatic organisms compared to the national observation surveys using multiple fishing nets and laboratory extraction/detection using a benchtop qPCR platform. Ultra-rapid methods from DNA collection to detection are still not well developed (1), especially for environmental DNA (eDNA) analysis, which uses water or soil samples to track the presence of target species (2, 3) . Here, we developed a new innovative method for the field processing of eDNA samples and measurements using an ultra-rapid mobile PCR platform (hereafter, mobile PCR) to reduce the measurement time to 30 min and maintain high detectability of aquatic organisms. We compared the on-site eDNA measurement to the laboratory extraction and detection using a benchtop qPCR platform and the national survey to confirm the performance. Our ultra-rapid on-site eDNA extraction and measurement method using mobile PCR successfully detected the eDNA of H.	./cache/cord-103417-2uinislh.txt	./txt/cord-103417-2uinislh.txt