id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103448-xa5zgkd3	Adachi, Hiroaki	Jurassic NLR: conserved and dynamic evolutionary features of the atypically ancient immune receptor ZAR1	2020-10-12		.txt	text/plain	9758	484	48	The phylogenetic tree was generated in MEGA7 by the neighbour-joining method using NB-ARC domain sequences of ZAR1-like proteins identified from the prior BLAST searches and 1019 NLRs identified from 6 representative plant species, taro, stout camphor, columbine, tomato, sugar beet and Arabidopsis. The overall conservation of the 120 ZAR1 orthologs enabled us to perform phylogenetic analyses using the full-length protein sequence and not just the NB-ARC domain as generally done with NLRs ( ZAR1 phylogenetic tree with well-supported branches that generally mirrored established phylogenetic relationships between the examined plant species (Smith and Brown, 2018; Chaw et al., 2019) . Taken together, based on the conserved motifs depicted in Figure 3A , we propose that angiosperm ZAR1 orthologs share the main functional features of Arabidopsis ZAR1: 1) effector recognition via RLCK binding, 2) remodelling of intramolecular interactions via ADP/ATP switch, 3) oligomerisation via the NBD-NBD interface and 4) α1 helix/MADA motifmediated activation of hypersensitive cell death.	./cache/cord-103448-xa5zgkd3.txt	./txt/cord-103448-xa5zgkd3.txt