id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103563-7a3wdduq	Nunez-Bajo, Estefania	Ultra-Low-Cost Integrated Silicon-based Transducer for On-Site, Genetic Detection of Pathogens	2020-03-25		.txt	text/plain	4535	211	44	Unlike other silicon-based technologies, TriSilix can be produced at wafer-scale in a standard laboratory; we have developed a series of methodologies based on metal-assisted chemical (wet) etching, electroplating, thermal bonding and laser-cutting to enable a cleanroom-free low-cost fabrication that does not require processing in an advanced semiconductor foundry. To create an ultra-low-cost device, the architecture proposed exploits the intrinsic properties of silicon and integrates three modes of operation in a single chip: i) electrical (Joule) heater, ii) temperature sensor (i.e. thermistor) with a negative temperature coefficient that can provide the precise temperature of the sample solution during reaction and iii) electrochemical sensor for detecting target NA. Using TriSilix, the sample solution can be maintained at a single, specific temperature (needed for isothermal amplification of NA such as Recombinase Polymerase Amplification (RPA) or cycled between different temperatures (with a precision of ±1.3°C) for Polymerase Chain Reaction (PCR) while the exact concentration of amplicons is measured quantitatively and in real-time electrochemically.	./cache/cord-103563-7a3wdduq.txt	./txt/cord-103563-7a3wdduq.txt