id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103946-c5i8btp7	Li, Maohua	Next generation of anti-PD-L1 Atezolizumab with better anti-tumor efficacy in vivo	2020-07-01		.txt	text/plain	4380	216	51	Our data shown that insertion of GGGS, without altering the anti-PD-L1 antibody affinity and inhibitory activity, completely abolished the ADCC activity, as same as Atezolizumab. Based on the structural information acquired from different antibodies in Protein data bank (e.g., PDB ID: 1IGT), we hypothesized that an insertion of a short but very flexible sequence in the hinge region or somewhere upstream of the glycosylation site of N297 may cut off the stress transmission signal between the Fv and Fc domain. Clearly, the insertion of GGGS between G237 and G238 of human IgG1 heavy chain showed no significant negative impact on antibody's affinity and inhibitory activity. Our data demonstrated that the insertion of GGGS in the hinge regions of human IgG1 could abolish their ADCC activities completely without concering negative impact on antibody affinities, inhibitory activities, expression levels, stabilities or immunogenicity. For those well-known antibody drugs, such as Genentech's aglycosylated anti-PD-L1 Atezolizumab, we demonstrated that inserting GGGS in the hinge regions of human IgG1 Fc could remove the ADCC activities completely.	./cache/cord-103946-c5i8btp7.txt	./txt/cord-103946-c5i8btp7.txt