id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103964-k6jnv87v	Friedl, Jana	More than just a ticket canceller: The mitochondrial processing peptidase matures complex precursor proteins at internal cleavage sites	2020-07-03		.txt	text/plain	2329	150	52	title: More than just a ticket canceller: The mitochondrial processing peptidase matures complex precursor proteins at internal cleavage sites After import into mitochondria, these targeting signals are cleaved off by the mitochondrial processing peptidase MPP, giving rise to shorter mature proteins. Arg5,6 is synthesized as a polyprotein precursor that is imported into the mitochondrial matrix and subsequently separated into two distinct enzymes that function in arginine biogenesis. Our data suggest that, in addition to its role as "ticket canceller" for the removal of presequences, MPP exhibits a second, widely conserved activity as internal processing peptidase for complex mitochondrial precursor proteins. We conclude that Arg5,6 is imported into the mitochondrial matrix 104 via the presequence pathway and cleaved into separate polypeptides inside mitochondria. Incubation of radiolabeled Arg5,6 precursor protein with MPP resulted in the formation of smaller 124 fragments whose size perfectly matched those that were generated after import into isolated 125 mitochondria (Fig. 1E ).	./cache/cord-103964-k6jnv87v.txt	./txt/cord-103964-k6jnv87v.txt