id author title date pages extension mime words sentences flesch summary cache txt cord-104031-vodwptdo Altshuler, Anna Capturing limbal epithelial stem cell population dynamics, signature, and their niche 2020-07-01 .txt text/plain 10255 580 53 Tremendous efforts have been made by many research groups to discover markers for the identification of LSCs. Keratin 15 (Krt15) 33 41 , C/EBPdelta and BMI1 42 , ABCB5 43 , ABCG2 44 and P63 45 represent a partial list of genes proposed to identify LSCs. Recently, we have shown that the Krt15-GFP transgene (green fluorescent protein coding gene under the promoter of Krt15) labeled a discrete population of murine LSCs. Krt15-GFP + basal limbal epithelial cells were located at close proximity to the site of corneal regeneration origin, as evident by lineage tracing of Krt14 + cells 33 . In silico analysis revealed discrete cell states in the corneal epithelial lineage Cell filtering and unbiased clustering were performed with R package Seurat 46 revealing 11 cell populations displaying a markedly distinct signature of gene expression (Fig. 1b, S1b) . ./cache/cord-104031-vodwptdo.txt ./txt/cord-104031-vodwptdo.txt