id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-104081-a3fx8tyd	Tang, Tiffany	Proteolytic activation of the SARS-CoV-2 spike S1/S2 site: a re-evaluation of furin cleavage	2020-10-05		.txt	text/plain	4004	206	57	The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uses its spike (S) protein to mediate viral entry into host cells. Our results demonstrate that S1/S2 pre-cleavage is essential for plasma membrane entry into Calu-3 cells, a model lung epithelial cell line, but not for endosomal entry Vero E6 cells, a model cell culture line, and that other proteases in addition to furin are responsible for processing SARS-CoV-2 S1/S2. dec-RVKR-CMK treatment had no significant impact on SARS-CoV S mediated infection of Vero E6 and Calu-3 cells (Figure 6A and 6B) , suggesting that dec-RVKR-CMK impacts on SARS-CoV-2 S is due to inhibiting the S1/S2 pre-cleavage and not due to some general effect on protein expression. Different residues in the SARS-CoV spike protein determine cleavage and activation by the host cell protease TMPRSS2 Cleavage Site in the Spike Protein of SARS-CoV-2 Is Essential for Infection of Human Lung Cells	./cache/cord-104081-a3fx8tyd.txt	./txt/cord-104081-a3fx8tyd.txt