id author title date pages extension mime words sentences flesch summary cache txt cord-104142-0nfprn2a Azmi, Maryam A. A laboratory module that explores RNA interference and codon optimization through fluorescence microscopy using Caenorhabditis elegans 2020-10-19 .txt text/plain 5438 276 50 In this laboratory module, students learn about RNA interference (RNAi) and codon optimization using the research organism Caenorhabditis elegans (C. elegans Understand the process of RNA interference and importance of codon optimization Learn basic microscopy techniques and image analysis Learn how to properly use the scientific method Enhance critical thinking skills Learning Objectives Students will be able to: Lab 1 and 2: Identify specific larval stages of C. elegans larvae using alkaline hypochlorite treatment Understand codon usage Formulate hypotheses and design a controlled experiment Lab 3 and 4: Acquire images using an epifluorescence microscope Effectively communicate results and formulate conclusions from data Describe what RNAi is and how it affects gene expression/activity Calculate mean fluorescent intensity from acquired fluorescence micrographs Perform statistical tests to determine the significance of results Generate publication quality figures and figure legends ./cache/cord-104142-0nfprn2a.txt ./txt/cord-104142-0nfprn2a.txt