id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-256607-wpywh8c9	Itokawa, Kentaro	Disentangling primer interactions improves SARS-CoV-2 genome sequencing by the ARTIC Network’s multiplex PCR	2020-06-01		.txt	text/plain	1415	76	59	The resulting primer set, version N1 (NIID-1), exhibits improved overall coverage compared to the ARTIC Network's original (V1) and modified (V3) primer set. Alongside with this modification, the ARTIC Network itself released another 108 modified version of primer set known as V3 in 24 th March 2020 (Loman and Quick 2020) after replacement for the same clinical sample (previously deposited to GISAID with ID EPI_ISL_416596, Ct=28.5, 1/25 input per reaction). Fig 3 Abundance of 98 amplicons at 8 different annealing/extension temperatures with the three different primer sets on a same clinical sample (previously deposited to GISAID with ID EPI_ISL_416584, Ct=16, 1/300 input per reaction). The orange lines and points indicate the abundances of amplicons whose primers were not modified but predicted to be eliminated the adverse primer interactions in the N1 primer set.	./cache/cord-256607-wpywh8c9.txt	./txt/cord-256607-wpywh8c9.txt