id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-261855-qpbgq5d8	Walker, Susanne N.	SARS-CoV-2 assays to detect functional antibody responses that block ACE2 recognition in vaccinated animals and infected patients	2020-06-18		.txt	text/plain	2533	166	57	title: SARS-CoV-2 assays to detect functional antibody responses that block ACE2 recognition in vaccinated animals and infected patients Here we describe a rapid serological diagnostic assay for determining antibody receptor blocking and demonstrate the broad utility of the assay by measuring the antibody functionality of sera from small animals and non-human primates immunized with an experimental SARS-CoV-2 vaccine and using sera from infected patients. Here, we describe a competition ELISA assay and a SPR 100 assay developed to rapidly detect ACE2 receptor blocking antibodies in IgGs and sera of 101 vaccinated mice, guinea pigs, rabbits and non-human primates, as well as, human samples (Fig. 1B) . Next, using Enzyme-Linked Immunosorbent Assays (ELISAs) we immobilized full-length 120 SARS-CoV-2 spike protein (containing both the S1 and S2 subunits) and incubated a dilution 121 series of ACE2-IgHu (Fig. 1D) . The proof-of-concept competition ELISA displayed a full blocking curve, so we sought to utilize 140 this assay for animals immunized with SARS-CoV-2 spike protein.	./cache/cord-261855-qpbgq5d8.txt	./txt/cord-261855-qpbgq5d8.txt