id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-262602-on0w55x0	Muruato, Antonio E.	A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation	2020-05-22		.txt	text/plain	880	53	53	Therefore, a high-throughput assay to measure SARS-CoV-2 neutralizing antibodies is urgently needed for COVID-19 serodiagnosis, convalescent plasma therapy, and vaccine development. Here we report on a fluorescence-based SARS-CoV-2 neutralization assay that detects SARS-CoV-2 neutralizing antibodies in COVID-19 patient specimens and yields comparable results to plaque reduction neutralizing assay, the gold standard of serological testing. To validate the reporter virus neutralization results, we performed the conventional 6 4 PRNT on the same set of patient specimens. The results demonstrate that when diagnosing 6 8 patient specimens, the reporter virus assay delivers neutralization results comparable to the 6 9 PRNT assay, the gold standard of serological testing. Next, we evaluated the specificity of reporter neutralization assay using potentially cross-7 1 reactive sera and interfering substances (Table 1) . Nevertheless, the 1 0 3 mNeonGreen reporter assay offers a rapid, high-throughput platform to test COVID-19 patient 1 0 4 sera not previously available.	./cache/cord-262602-on0w55x0.txt	./txt/cord-262602-on0w55x0.txt