id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-263780-8jejswuk	Wang, Nan	Chloroquine and hydroxychloroquine as ACE2 blockers to inhibit viropexis of 2019-nCoV Spike pseudotyped virus	2020-08-14		.txt	text/plain	1584	103	66	title: Chloroquine and hydroxychloroquine as ACE2 blockers to inhibit viropexis of 2019-nCoV Spike pseudotyped virus Purpose The objective of this study is to investigate whether CQ and HCQ could be ACE2 blockers and used to inhibit 2019-nCoV virus infection. We further analyzed the binding character of CQ and HCQ to ACE2 by molecular docking and surface plasmon resonance (SPR) assays, 2019-nCoV spike pseudotyped virus was also used to observe the viropexis effect of CQ and HCQ in ACE2h cells. Conclusions CQ and HCQ both inhibit the entrance 2019-nCoV into cells by blocking the binding of the virus with ACE2. In this study, we found that CQ and HCQ can antagonize ACE2 and inhibit the entry of 2019-nCoV 103 spike pseudotyped virus into ACE2 expressed HEK293T cells (ACE2 h cells). It can be concluded that the toxicity 216 of HCQ was higher than that of CQ on ACE2 h cells at different time points at the same 217 concentrations (Figure 1C) .	./cache/cord-263780-8jejswuk.txt	./txt/cord-263780-8jejswuk.txt