id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-267845-18hb5ndr	Resende, Paola Cristina	SARS-CoV-2 genomes recovered by long amplicon tiling multiplex approach using nanopore sequencing and applicable to other sequencing platforms	2020-05-01		.txt	text/plain	1616	89	48	Here, we describe three protocols using a unique primer set designed to recover long reads of SARS-CoV-2 directly from total RNA extracted from clinical samples. Despite those limitations, we developed a sequencing protocol that successfully obtained whole genomes from SARS-CoV-2 positive samples referred to the National Reference laboratory at FIOCRUZ in Brazil. The tiling amplicon multiplex PCR method has been previously used for virus sequencing directly from clinical samples to obtain consensus genome sequences (3). Here, we describe three protocols using a primer set designed to sequence SARS-CoV-2 directly from total RNA extracted from clinical samples, which were initially diagnosed using real-time RT-PCR (7, 8) . Here we introduce a versatile sequencing protocol to recover the complete SARS-CoV-2 genome based on reverse transcription plus an overlapping long amplicon multiplex PCR strategy, and associated with pipelines to report the data, and recover the consensus files. Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples	./cache/cord-267845-18hb5ndr.txt	./txt/cord-267845-18hb5ndr.txt