id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-271971-rstmd0va	Matsumura, Yasufumi	Comparison of 12 molecular detection assays for SARS-CoV-2	2020-06-25		.txt	text/plain	1203	74	60	We compared 12 molecular diagnostic assays, including 8 commercial kits using 155 respiratory samples (65 nasopharyngeal swabs, 45 oropharyngeal swabs, and 45 sputum) collected at 2 Japanese hospitals. Real-time RT-PCRs 54 were performed using N1, N2, and RNaseP (RP) internal control assays developed by the (4) (with/without EAV), N and E assays developed by Charité in Germany (1) (Corman) 59 with TaqPath™ 1-Step RT-qPCR Master Mix, CG (Thermo Fisher Scientific). In this study, to 79 ensure the presence of SARS-CoV-2 RNA and to avoid false-positives, a sample was 80 defined as positive when positive test results were obtained for more than one genetic 81 locus and assay and the others were defined as negative. 166 Different from multiplex assays that incorporate internal controls such as Roche, Thermo, 167 or BGI kits, this approach needs extra reagents, time, and space in a reaction plate but 168 can be combined with other in-house assays (NIID N2 or Corman E) without any 169 modification.	./cache/cord-271971-rstmd0va.txt	./txt/cord-271971-rstmd0va.txt