id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-273083-xrydkiu4	Pahmeier, Felix	A versatile reporter system to monitor virus infected cells and its application to dengue virus and SARS-CoV-2	2020-09-01		.txt	text/plain	999	64	49	Here, we describe the generation and characterization of a reporter system to visualize dengue virus and SARS-CoV-2 replication in live cells. The system is based on viral protease activity causing cleavage and nuclear translocation of an engineered fluorescent protein that is expressed in the infected cells. Here we describe a reporter system that takes advantage of virus-encoded proteases that are expressed in infected cells to cleave an ER-anchored fluorescent protein fused to a nuclear localization sequence. Using this system, we demonstrate reliable reporting activity for two major human pathogens from the Flaviviridae and the Coronaviridae families: dengue virus and SARS-CoV-2. In order to generate a reporter system that can specifically indicate virus infection, we 219 designed a construct expressing a GFP fusion protein that could selectively be cleaved 220 by viral proteases. However, since no fluorescent 304 protein coding sequence is incorporated into the construct, expression of the DENV 305 polyprotein cannot be followed by live cell imaging.	./cache/cord-273083-xrydkiu4.txt	./txt/cord-273083-xrydkiu4.txt