id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-279576-wt4crton	Fajardo, Álvaro	Evaluation Of SYBR Green Real Time PCR For Detecting SARS-CoV-2 From Clinical Samples	2020-05-13		.txt	text/plain	4842	263	54	Several methods based on real time reverse transcription polymerase chain reaction (RT-qPCR) for the detection of SARS-CoV-2 genomic RNA have been developed. The aim of the study was to set up an alternative molecular protocol to detect SARS-CoV-2 from clinical samples, without the need of TaqMan probes or post-PCR steps (i.e. gel electrophoresis), which can be implemented in case of difficulties to get specific reagents or kits because of the current pandemic situation. In order to select an appropriate amount of control vector to use in the comparison between the two real time qPCR methods, we prepared plasmids dilutions (107, 106, 105 and 104 copies/μL) and assayed them following both protocols: the probe-based One Step RT-qPCR developed by the University of Hong Kong Poon et al. The amplification data for the SYBR Green-based qPCR protocol showed that the ORF1b-nsp14 region was correctly amplified for all SARS-CoV-2 positive samples (1 to 7) (Fig. 3) .	./cache/cord-279576-wt4crton.txt	./txt/cord-279576-wt4crton.txt