id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-282795-kje7rn57	Zheng, Yue	Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions	2020-09-21		.txt	text/plain	487	36	58	To easily and rapidly measure titers of neutralizing antibodies in serum or plasma, we developed pseudovirion particles composed of the spike glycoprotein of SARS-CoV-2 incorporated onto murine leukemia virus capsids and a modified minimal MLV genome encoding firefly luciferase. Pseudotyped MLV viruses were tested on HEK293FT, HEK293T-ACE2, Huh7 and SupT1 cells. To test for specificity of neutralization, we asked whether neutralizing antibodies from SARSCoV-2 patients would exhibit cross-reactivity against a pseudotype expressing SARS-CoV-1 ( Figure 112 4). Characterization of 162 spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with 163 SARS-CoV Veesler D: Structure, Function, and 165 Antigenicity of the SARS-CoV-2 Spike Glycoprotein The 167 D614G mutation in the SARS-CoV-2 spike protein reduces S1 shedding and increases 168 infectivity High-efficiency gene 170 transfer into CD34+ cells with a human immunodeficiency virus type 1-based retroviral 171 vector pseudotyped with vesicular stomatitis virus envelope glycoprotein G Pseudotyping Viral Vectors With Emerging Virus Envelope 177 Proteins	./cache/cord-282795-kje7rn57.txt	./txt/cord-282795-kje7rn57.txt