id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-285159-gytebbua	Eydoux, Cecilia	A Fluorescence-based High Throughput-Screening assay for the SARS-CoV RNA synthesis complex	2020-07-07		.txt	text/plain	3603	215	59	Here, we report the use of a purified and highly active SARS-CoV replication/transcription complex (RTC) to set-up a high-throughput screening of Coronavirus RNA synthesis inhibitors. Principle of SARS-CoV RNA synthesis detection by a fluorescence-based high throughput screening assay Highlights A new SARS-CoV non radioactive RNA polymerase assay is described The robotized assay is suitable to identify RdRp inhibitors based on HTS -A new SARS-CoV non radioactive RNA polymerase assay is described -The robotized assay is suitable to identify RdRp inhibitors based on HTS the RdRp core nsp12 and shown to confer full activity and processivity to nsp12 (Subissi et al., 2014) . Picogreen kinetic assay was based on polymerase activity of SARS nsp12 in complex with nsp7L8, which catalyzed the reaction using a poly (A) template and uridine triphosphate (UTP).	./cache/cord-285159-gytebbua.txt	./txt/cord-285159-gytebbua.txt