id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-288705-f3zqhpx1	Slaine, Patrick	Thiopurines activate an antiviral unfolded protein response that blocks viral glycoprotein accumulation in cell culture infection model	2020-10-01		.txt	text/plain	5892	325	45	title: Thiopurines activate an antiviral unfolded protein response that blocks viral glycoprotein accumulation in cell culture infection model Selective disruption of IAV glycoprotein processing and accumulation by 6-TG and 6-TGo correlated with unfolded protein response (UPR) activation and HA accumulation could be partially restored by the chemical chaperone 4-phenylbutyrate (4PBA). Thiopurines inhibited replication of the human coronavirus OC43 (HCoV-OC43), which also correlated with UPR/ISR activation and diminished accumulation of ORF1ab and nucleocapsid (N) mRNAs and N protein, which suggests broader disruption of coronavirus gene expression in ER-derived cytoplasmic compartments. Our results suggest that 406 the effects are unlikely to be mediated through DNA or RNA incorporation of 6-TG because 1) 407 replicative stress does not specifically induce UPR; 2) among viral proteins, glycoprotein 408 accumulation and processing was preferentially disrupted; 3) messenger RNA levels of HA and 409 NA were not affected.	./cache/cord-288705-f3zqhpx1.txt	./txt/cord-288705-f3zqhpx1.txt