id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-295560-0rpguepv	Yan, Kexin	Simple rapid in vitro screening method for SARS-CoV-2 anti-virals that identifies potential cytomorbidity-associated false positives	2020-10-14		.txt	text/plain	1883	99	55	The assay clearly illustrated the anti-viral activity of remdesivir, a drug known to inhibit SARS-CoV-2 replication. Here we describe a simple rapid bioassay for drug screening using Vero 20 E6 cells and inhibition of cytopathic effects (CPE) measured using crystal violet staining. A key refinement involves a simple growth assay to identify drug concentrations that 23 cause cellular stress or "cytomorbidity", as distinct from cytotoxicity or loss of viability. A key refinement involves a simple growth assay to identify drug concentrations that 23 cause cellular stress or "cytomorbidity", as distinct from cytotoxicity or loss of viability. Thus a drug that has no specific anti-viral activity, but that 52 is able to induce cellular stress, may therefore inhibit virus replication non-specifically and generate 53 a potential false positive in the screening assay. A simple rapid bioassay for screening drugs for potential antiviral activity against SARS-CoV-2 72 is to determine whether the drug can inhibit virus-induced cytopathic effects (CPE) in Vero E6 cells.	./cache/cord-295560-0rpguepv.txt	./txt/cord-295560-0rpguepv.txt