id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-295765-c7o2ukm6	Silvas, Jesus A.	Inhibitors of VPS34 and lipid metabolism suppress SARS-CoV-2 replication	2020-07-20		.txt	text/plain	2234	145	53	VPS34 inhibitors, Orlistat and Triacsin C inhibited virus growth in Vero E6 cells and in the human airway epithelial cell line Calu-3, acting at a post-entry step in the virus replication cycle. As SARS-CoV-2 replication 174 damages the cell monolayer, impedance measurements decrease over time, providing a detailed 175 assessment of infection kinetics. Based on the toxicity window of 1-20 221 h.p.t. determined with the VPS34 inhibitors, neither Triacsin C nor Orlistat induced early 222 cytotoxic effects, even at the highest concentrations of 50uM and 500uM, respectively ( Figure 223 3A and 3C). Here, we demonstrate that two VPS34 inhibitors, Orlistat, and Triacsin C each have clear effects 308 on SARS-CoV-2 replication and the morphology of viral replication centers. In contrast, the compounds did not exhibit any activity against 384 SARS-CoV-2 in Vero E6 cells whereas Triacsin C did.	./cache/cord-295765-c7o2ukm6.txt	./txt/cord-295765-c7o2ukm6.txt