id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-298326-f5q7j3iu	Nick, Benjamin C.	Identification of a critical horseshoe-shaped region in the nsp5 (Mpro, 3CLpro) protease interdomain loop (IDL) of coronavirus mouse hepatitis virus (MHV)	2020-06-19		.txt	text/plain	2755	118	54	In this study, we describe the identification of an essential, conserved horseshoe-shaped region in the nsp5 interdomain loop (IDL) of mouse hepatitis virus (MHV), a common coronavirus replication model. Structural modeling and sequence analysis of these sites in other coronaviruses, including all 7 human coronaviruses, suggests that the identified structure and sequence of this horseshoe regions is highly conserved and may represent a new, non-active-site regulatory region of the nsp5 (3CLpro) protease to target with coronavirus inhibitors. To evaluate whether any of 207 the recovered MHV nsp5 IDL mutants may exhibit a temperature-sensitive phenotype, we 208 performed an efficiency of plating (EOP) analysis by comparing the titers of each IDL virus by 209 plaque assay determined at a physiologic (37°C) and elevated temperature (40°C) (Fig. 3A) .	./cache/cord-298326-f5q7j3iu.txt	./txt/cord-298326-f5q7j3iu.txt