id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-300707-k9uk14b3	Bouwman, Kim M.	Multimerization- and glycosylation-dependent receptor binding of SARS-CoV-2 spike proteins	2020-09-04		.txt	text/plain	2328	176	59	Here we created monomeric and trimeric fluorescent RBD proteins, derived from adherent HEK293T, as well as in GnTI mutant cells, to analyze the effect of complex vs high mannose glycosylation on receptor binding. Our results show that fully glycosylated trimeric RBD proteins are attractive to analyze receptor binding and explore ACE2 expression profiles in tissues. The results demonstrate 104 that fully glycosylated trimeric SARS-CoV-2 RBD proteins reveal the 105 differences in ACE2 expression between cell cultures and tissue sections. A similar trend of binding intensities was observed for 214 monomeric, trimeric, and different N-glycosylated SARS-CoV-RBD proteins 215 fused to mOrange2 ( Fig S3A) . 226 227 To confirm our observations of different binding on tissues, we quantified the 228 intensities of the ACE2 antibody and SARS-CoV-1 and -2 RBD proteins, except 229 for the monomeric GnTI derived proteins as these were almost at the 230 background ( Fig 4D) .	./cache/cord-300707-k9uk14b3.txt	./txt/cord-300707-k9uk14b3.txt