id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-303399-s1hbpvn7	Straus, Marco R.	SPINT2 inhibits proteases involved in activation of both influenza viruses and metapneumoviruses	2019-08-31		.txt	text/plain	6424	466	64	SPINT2 treatment resulted in the cleavage and fusion inhibition of full-length influenza A/CA/04/09 (H1N1) HA, A/Aichi/68 (H3N2) HA, A/Shanghai/2/2013 (H7N9) HA and HMPV F when activated by trypsin, recombinant matriptase or KLK5. https://doi.org/10.1101/752592 doi: bioRxiv preprint vivo situation and requires validation by expressing the full-length fusion proteins in a cell culture model 3 to test cleavage and cleavage inhibition of the respective protease (39). To test SPINT2-mediated cleavage inhibition of full-length HA we expressed the HAs of A/CA/04/09 9 (H1N1), A/x31 (H3N2) and A/Shanghai/2/2013 (H7N9) in 293T cells and added recombinant matriptase or 10 KLK5 protease that were pre-incubated with 10nM or 500nM SPINT2. To understand whether SPINT2 was able to inhibit or reduce the growth of virus in a cell culture 20 model over the course of 48 hours we transfected cells with human TMPRSS2 and human matriptase, two 21 major proteases that have been shown to be responsible for the activation of distinct influenza A subtype 22	./cache/cord-303399-s1hbpvn7.txt	./txt/cord-303399-s1hbpvn7.txt