id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-303868-aes92l6s	Steffen, Tara L.	The receptor binding domain of SARS-CoV-2 spike is the key target of neutralizing antibody in human polyclonal sera	2020-08-22		.txt	text/plain	6098	300	46	In this study, we identify the spike protein subunits that contain antigenic determinants and examine the neutralization capacity of polyclonal sera from a cohort of patients that tested qRT-PCR-positive for SARS-CoV-2. In this study, we identify the spike protein subunits that contain antigenic determinants and examine the neutralization capacity of polyclonal sera from a cohort of patients that tested qRT-PCR-positive for SARS-CoV-2. This suggests that polyclonal antibody binding to the RBD domain of the spike protein represents the key target of neutralizing antibody to SARS-CoV-2 after natural infection. Most importantly, our antigen-specific antibody depletion approach demonstrated that the RBD domain of the spike protein is responsible for 70% +/-18.9% of the human polyclonal neutralizing antibody activity to spike after natural SARS-CoV-2 infection. Although our study shows that the dominant target of IgG neutralizing antibody response after natural SARS-CoV-2 infection is the RBD domain of the spike protein, we have evaluated a limited number (n=10) of patients by antigen-specific antibody depletion.	./cache/cord-303868-aes92l6s.txt	./txt/cord-303868-aes92l6s.txt