id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-305496-t8ykkekl	Stone, E. Taylor	Characterization of cells susceptible to SARS-COV-2 and methods for detection of neutralizing antibody by focus forming assay	2020-08-21		.txt	text/plain	7227	391	60	One such tool for evaluating neutralizing antibody response is a 88 plaque/focus neutralization reduction test (PRNT/FRNT), which evaluates the ability of polyclonal 89 sera samples to prevent or reduce infection of a cell monolayer in vitro. We examined the impact of cell density on foci formation for both Vero WHO and Vero E6 cells 144 by plating identical dilutions of SARS-CoV-2 virus stocks on 96-well plates seeded with differing 145 numbers of WHO or E6 cells (3 × 104, 1.5 × 104 or 3 × 104 cells/well) one day prior to infection 146 of the cell monolayer. To determine the optimal time frame for infection of SARS-CoV-2 on a Vero WHO cell 172 monolayer to form individual foci, we tested a variety of incubation times. The FFA relies on an immunostaining protocol of an infected cell monolayer in order to 197 quantify infectious virus titer and is therefore dependent upon SARS-CoV-2-specific antibody 198	./cache/cord-305496-t8ykkekl.txt	./txt/cord-305496-t8ykkekl.txt