id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-307242-e20gtx0z	Jegouic, Sophie M.	Recombinant SARS-CoV-2 spike proteins for sero-surveillance and epitope mapping	2020-05-22		.txt	text/plain	3454	177	52	Similar western blot analysis of total protein extracts following induction of logarithmic phase E.coli cultures with IPTG confirmed the expression of His-tagged S antigen of the predicted molecular weight in all cases ( Figure 3 , upper panel). Nevertheless, we found that S protein fragments prepared for gel electrophoresis using non-reducing loading buffer could be used successfully for epitope mapping of 2 S reactive monoclonal antibodies, 3G9, an unpublished mouse mAb generated to SARS S, and CR3022, a human mAb also isolated originally to SARS [19] but shown to cross-react with SARS-CoV-2. To provide an additional level of validation and to add epitope specificity to the data, 2 of the sera scoring positive by S1 ELISA were used as probes on western blots using full length S expressed in insect cells (cf. Optimization of the Production Process and Characterization of the Yeast-Expressed SARS-CoV Recombinant Receptor-Binding Domain ( RBD219-N1 ), a SARS Vaccine Candidate	./cache/cord-307242-e20gtx0z.txt	./txt/cord-307242-e20gtx0z.txt