id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-312228-wbyqmvhs	Xiao, Minfeng	Multiple approaches for massively parallel sequencing of HCoV-19 (SARS-CoV-2) genomes directly from clinical samples	2020-03-19		.txt	text/plain	3236	194	56	Here we illustrate the application of amplicon and hybrid capture (capture)-based sequencing, as well as ultra-high-throughput metatranscriptomic (meta) sequencing in retrieving complete genomes, inter-individual and intra-individual variations of HCoV-19 from clinical samples covering a range of sample types and viral load. 90 Therefore, we aim to comprehensively compare the sensitivity, inter-individual (variant) and 91 intra-individual (iSNV) accuracy, and other general features of different approaches by sys-92 tematically utilizing ultra-high-throughput metatranscriptomic, hybrid capture-based, and 93 amplicon-based sequencing approaches to obtain genomic information of HCoV-19 from 94 serial dilutions of a cultured isolate and directly from clinical samples. We assessed the correlations between the HCoV-19 genome copies per mL in diluted 396 samples of cultured isolates and the minimum amount of sequencing data for amplicon-397 and capture-based methods using Pearson correlation coefficient (R) with the function 398 scatter from the R package ggpubr (v3.6.1) 38 .	./cache/cord-312228-wbyqmvhs.txt	./txt/cord-312228-wbyqmvhs.txt