id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-312414-g5px0b65	Takagi, Akira	An immunodominance hierarchy exists in CD8+ T cell responses to HLA-A*02:01-restricted epitopes identified from the non-structural polyprotein 1a of SARS-CoV-2	2020-09-19		.txt	text/plain	4076	230	61	As shown in Fig. 2 , the intracellular cytokine staining (ICS) assay showed that 173 significant numbers of IFN--producing CD8+ T cells were elicited in mice immunized 174 with 18 liposomal peptides including pp1a-38, -52, -84, -103, -445, -597, -641, -1675, 175 -2785, -2884, -3083, -3403, -3467, -3583, -3662, -3710, -3732, and -3886, revealing that 176 these 18 peptides are HLA-A*02:01-restricted CTL epitopes derived from SARS-CoV-2 177 pp1a. However, any of 18 185 epitopes are not found in the amino acid sequence of either MERS-CoV or the four 186 common cold human coronaviruses involving HCoV-OC43, In the 18 positive peptides, 10 peptides including pp1a-38, -84, -641, -1675, -2884, 189 -3467, -3583, -3662, -3710, and -3732 were selected for the following analyses because 190 of the high ratios of IFN- + cells in CD8 + T cells (Fig. 2) . At first glance, the graphs of CD107a ( Taken together, 10 peptides differed significantly in their ability to induce 226 SARS-CoV-2 pp1a-specific CTLs when mice were immunized with the mixture of 10 227 peptides in liposomes.	./cache/cord-312414-g5px0b65.txt	./txt/cord-312414-g5px0b65.txt