id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-314072-av3r7it7	Liu, Zhuoming	Landscape analysis of escape variants identifies SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization	2020-11-08		.txt	text/plain	1105	74	54	title: Landscape analysis of escape variants identifies SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization To define the immune-mediated mutational landscape in S protein, we used a VSV-eGFP-SARS-CoV-2-S chimeric virus and 19 neutralizing monoclonal antibodies (mAbs) against the receptor binding domain (RBD) to generate 48 escape mutants. Although each mAb had unique resistance profiles, many shared residues within an epitope, as several variants were resistant to multiple mAbs. Remarkably, we identified mutants that escaped neutralization by convalescent human sera, suggesting that some humans induce a narrow repertoire of neutralizing antibodies. By comparing the antibody-mediated mutational landscape in S protein with sequence variation in circulating SARS-CoV-2 strains, we identified single amino acid substitutions that could attenuate neutralizing immune responses in some humans. To select for SARS-CoV-2 S variants that escape neutralization, we used VSV-SARSas we isolated this mutation alone, and acquisition of the L517R substitution appeared to 141 increase viral fitness as judged by plaque morphology (Fig S2) .	./cache/cord-314072-av3r7it7.txt	./txt/cord-314072-av3r7it7.txt