id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-318444-sgm24q1i	Walter, Justin D.	Sybodies targeting the SARS-CoV-2 receptor-binding domain	2020-05-16		.txt	text/plain	5902	416	49	Two independently prepared RBD constructs were used for in vitro sybody selections, and resulting single clones that could bind the full spike ectodomain were sequenced, expressed, and purified. Six unique sybodies show favorable binding affinity to the SARS-CoV-2 spike, and five of these were also found to substantially attenuate the interaction between the viral RBD and human ACE2. While this purified pre-fusion spike (PFS) had not yet been available for binder selections and characterization by grating-coupled interferometry, it was used to conduct ELISAs in order to identify selected sybodies which recognize the RBD in the pre-fusion context (see below). Since virulence of SARS-CoV-2 is dependent on the ability of the viral RBD to bind to human ACE2 (hACE2), we sought to determine which of the 57 selected sybodies that were well-behaved upon purification could inhibit interaction between the isolated RBD and purified hACE2.	./cache/cord-318444-sgm24q1i.txt	./txt/cord-318444-sgm24q1i.txt