id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-319100-3gdawhfn	Kirkland, P.D.	The impact of viral transport media on PCR assay results for the detection of nucleic acid from SARS-CoV-2 and other viruses	2020-06-10		.txt	text/plain	4624	204	49	authors: Kirkland, P.D.; Frost, M.J. title: The impact of viral transport media on PCR assay results for the detection of nucleic acid from SARS-CoV-2 and other viruses Also of concern are recommendations (3, 4) to include foetal bovine serum (fbs) as a source of protein to enhance the stabilising properties of VTMs. This report documents observations of the adverse impact of certain VTMs on real time reverse transcription PCR (qRT-PCR) assays for the detection of SARS-CoV-2 virus as well as on a Type A influenza virus and a herpesvirus and discuss the broader implications of the inclusion of foetal bovine serum as a protein supplement to VTMs. During the initial investigation, purified RNA from an Australian isolate (WMD DC1) of SARS-CoV-2 was supplied to the Elizabeth Macarthur Agriculture Institute (EMAI) by the Institute of Clinical Pathology and Medical Research (ICPMR), Westmead, New South Wales (NSW).	./cache/cord-319100-3gdawhfn.txt	./txt/cord-319100-3gdawhfn.txt