id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-321049-9ozn6il7	de Almeida, Paula Rodrigues	SARS-CoV2 quantification using RT-dPCR: a faster and safer alternative to assist viral genomic copies assessment using RT-qPCR	2020-05-01		.txt	text/plain	1308	73	49	title: SARS-CoV2 quantification using RT-dPCR: a faster and safer alternative to assist viral genomic copies assessment using RT-qPCR Narrower confidence intervals, indicating high quantification precision were obtained in 100 and 1000-fold serial dilution and RT-dPCR results were equivalent between different assays in the same dilution. Here, we present a fast, accurate and simple method of viral titration using QuantStudio 3D® microchip based RT-dPCR to titrate SARS-CoV2 genomic copies from controls to be used in RT-qPCR assays for diagnosis and research purposes. A dilution that results in approximately 200 to 2000 target copies in the final reaction usually presents better precision values. Results with precision values below 5% were selected to estimate quantity of SARS-CoV2 genomic copies based on RT-dPCR. RT-dPCR results of 10-fold serial dilution of SARS-CoV2 control using assays for three targets in the Nucleocapsid gene. These results indicate that these primer-probe assays are suitable for SARS-CoV2 quantification through RT-dPCR.	./cache/cord-321049-9ozn6il7.txt	./txt/cord-321049-9ozn6il7.txt