id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-323654-9nnjex9y	Ramachandran, Ashwin	Electric-field-driven microfluidics for rapid CRISPR-based diagnostics and its application to detection of SARS-CoV-2	2020-05-22		.txt	text/plain	1549	115	62	Unlike previous CRISPR diagnostic assays, we also use ITP for automated purification of target RNA from raw nasopharyngeal swab sample. We here combine this ITP purification with loop-mediated isothermal amplification, and the ITP-enhanced CRISPR assay to achieve detection of SARS-CoV-2 RNA (from raw sample to result) in 30 min for both contrived and clinical nasopharyngeal swab samples. We also demonstrated on-chip ITP extraction of total nucleic acids from raw clinical 175 positive and negative nasopharyngeal swab samples (Figs. We observed that ITP-extracted nucleic acids showed E gene amplification on positive qPCR-based detection approaches is provided in Table 1 . We propose here 520 the concept that such a system can integrate ITP-based nucleic acid extraction, 521 multiplexed isothermal amplification of target cDNA of N and E genes of SARS-CoV-2 522 and RNase P control, followed by ITP-CRISPR-based cDNA detection in three separate 523 channels using photodiodes.	./cache/cord-323654-9nnjex9y.txt	./txt/cord-323654-9nnjex9y.txt