id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-326337-s0fp5z1q	Chan, Kui K.	An engineered decoy receptor for SARS-CoV-2 broadly binds protein S sequence variants	2020-10-19		.txt	text/plain	4573	256	54	Deep mutagenesis of the isolated receptor-binding domain (RBD) by yeast surface display 44 has easily identified mutations in S that retain high expression and ACE2 affinity, yet are no longer bound 45 by monoclonal antibodies and confer resistance (19) . An alternative protein-based antiviral to monoclonal antibodies is to use soluble ACE2 (sACE2) as a 56 decoy to compete for receptor-binding sites on the viral spike (6, (22) (23) (24) (25) of diverse SARS-associated betacoronaviruses that use ACE2 for entry. The sequence 162 diversity observed among natural betacoronaviruses, which display high diversity at the ACE2 binding 163 site, is therefore replicated in the deep mutational scan, which predicts the SARS-CoV-2 spike tolerates 164 substantial genetic diversity at the receptor-binding site for function. From this accessible sequence 165 diversity SARS-CoV-2 might feasibly mutate to acquire resistance to monoclonal antibodies or 166 engineered decoy receptors targeting the ACE2-binding site.	./cache/cord-326337-s0fp5z1q.txt	./txt/cord-326337-s0fp5z1q.txt