id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-327711-welf0eb1	Zhou, Daming	Structural basis for the neutralization of SARS-CoV-2 by an antibody from a convalescent patient	2020-06-13		.txt	text/plain	4847	290	59	Cryo-EM analyses of the pre-fusion Spike incubated with EY6A Fab reveal a complex of the intact trimer with three Fabs bound and two further multimeric forms comprising destabilized Spike attached to Fab. EY6A binds what is probably a major neutralising epitope, making it a candidate therapeutic for COVID-19. A neutralisation test for EY6A based on quantitative PCR detection of virus in the supernatant bathing infected Vero E6 cells after 5 days of culture, showed a ~1000-fold reduction in virus signal (Methods, Extended Data Fig. 3 ) indicating that it is highly neutralising. To elucidate the epitope of EY6A, we determined the crystal structures of the deglycosylated SARS-CoV-2 RBD in complex with EY6A Fab alone and in a ternary complex incorporating a nanobody (Nb) which has been shown to compete with ACE2 (for data on a closely related Nb see Huo 2020, submitted), as a crystallisation chaperone.	./cache/cord-327711-welf0eb1.txt	./txt/cord-327711-welf0eb1.txt