id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-327912-wfjdxgxh	Swann, Heather	Minimal system for assembly of SARS-CoV-2 virus like particles	2020-08-24		.txt	text/plain	1695	98	59	Here we demonstrate that non-infectious SARS-CoV-2 virus like particles (VLPs) can be assembled by co-expressing the viral proteins S, M and E in mammalian cells. Non-infectious virus like particles (VLPs) displaying essential viral proteins can be used to study the structural properties of the SARS-CoV-2 virions and due to their maximum immunogenicity are also vaccine candidates 2, 3 . Similarly, expression of M, E and S proteins are shown to result in release of morphologically identical particles to wild type SARS-CoV virus 9, 10 . We then tested the structural integrity of the SARS-CoV-2 VLPs attached to dry glass using Atomic Force Microscopy (AFM), since SARS-CoV-2 virions have been reported to survive on solid surfaces in dry conditions for many hours 13 . SARS-CoV-2 M, S and E protein genes were identified from the full genome sequence of the virus 1 , these genes were then humanized and inserted in CMV driven mammalian expression vectors (see supplement for complete plasmid sequences).	./cache/cord-327912-wfjdxgxh.txt	./txt/cord-327912-wfjdxgxh.txt