id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-328636-1q71gjwt	Arora, Kajal	Multi-Antigenic Virus-like Particle of SARS CoV-2 produced in Saccharomyces cerevisiae as a vaccine candidate	2020-05-19		.txt	text/plain	2249	118	55	title: Multi-Antigenic Virus-like Particle of SARS CoV-2 produced in Saccharomyces cerevisiae as a vaccine candidate We hereby report recombinant co-expression of the three proteins (Spike, Envelope and Membrane) in a engineered Saccharomyces cerevisiae platform (D-Crypt™) and their self-assembly as Virus-like particle (VLP). In this study, we report the first successful self-assembly of the SARS CoV-2 VLP by co-expression of the S, E and M proteins in Saccharomyces cerevisiae expression platform. cerevisiae, recombinant construct for the expression of S and E protein, pYRE100_CSP_CEP_His and M protein, pYRI100_CMP_His construct were cotransformed into the host and the positive clones were selected on YNB-URA-LEU plates. Episomal constructs of S, E and M with His tag, pYRE100_CSP_His, pYRE100_CMP_His and pYRE100_CEP_His plasmid and host vector pYRE100 plasmid were transformed into proprietary protease deficient PYPD yeast expression strain using Lithium acetate/SS-DNA/PEG mediated protocol and transformants were selected over selective YNB Glucose minus URA plates.	./cache/cord-328636-1q71gjwt.txt	./txt/cord-328636-1q71gjwt.txt