id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-331680-qlzhtxs0	Goryachev, A.N.	Potential Opportunity of Antisense Therapy of COVID-19 on an in Vitro Model	2020-11-03		.txt	text/plain	4106	200	51	In accordance with the purpose of the study, the following tasks were set: -to select the nucleotide sequence of the virus that is supposed to be inhibited, -to carry out the synthesis of oligonucleotide, -to determine cytotoxicity and antiviral activity in an in vitro experiment on cell culture. The assessment of antiviral activity of the drug in addition to cytopathic action was also taken into account by reducing the infectious titer of the virus in the culture of Vero cells E6 according to PCR RNA SARS-CoV-2, determined by the threshold of the number of reaction cycles (cycle treshold, Ct) in various dilutions of the study drug. Determination of the antiviral efficacy of the antisense oligonucleotide according to the treatment scheme (administration of the drug 24 hours after infection) was taken into account by the decrease in the infectious titer of the virus in the culture of Vero E6 cells by the cytopathic effect.	./cache/cord-331680-qlzhtxs0.txt	./txt/cord-331680-qlzhtxs0.txt