id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-332134-88wfcc3y	Li, Tingting	A potent synthetic nanobody targets RBD and protects mice from SARS-CoV-2 infection	2020-09-24		.txt	text/plain	2051	158	57	title: A potent synthetic nanobody targets RBD and protects mice from SARS-CoV-2 infection Molecular mechanism for neutralization 157 Structure alignment of SR4-, MR17-and ACE2-RBD 4 showed that both sybodies 158 engage with RBD at the receptor-binding motif (RBM) ( Fig. 2A, 2B) . Taken together, SR4 169 and MR17, and probably MR3, neutralize SARS-CoV-2 by competitively blocking the For biparatopic fusion, we first identified two sybodies, namely LR1 and LR5 (Fig. 208 3A, 3B), that could bind RBD in addition to MR3 using the BLI assay. As LR5 showed 209 higher affinity and neutralization activity than LR1 (Fig. 1A) , we fused this non-210 competing sybody to the N-terminal of MR3 with various length of GS linkers ranging 211 from 13 to 34 amino acids (Extended Data Table S1 ). Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with 803	./cache/cord-332134-88wfcc3y.txt	./txt/cord-332134-88wfcc3y.txt