id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-332374-cbiw6yvb	Israeli, Ofir	Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction	2020-06-10		.txt	text/plain	1051	77	55	Very recently, two studies [6] [7] used a direct no-buffer RT-qPCR approach which identified > 90% of the tested clinical samples. In this study, we tested the diagnostic efficiency following thermal inactivation (65°C for 30min and 95°C for 10min) without addition of lysis buffers ("no buffer") or following lysis by three buffers (Virotype, QuickExtract and 2% Triton-X-100) and compared it to diagnosis after standard RNA extraction. Samples included buffers spiked with SARS-CoV-2, at concentrations 0.1-100,000 PFU/ml and 30 clinical samples, previously diagnosed as positive (20) and negative (10). The limit of detection was 1 PFU/ml: In this concentration samples in the no buffer mode and Virotype at 95°C were not detected, while the RNA extraction mode averaged the lowest critical threshold ( Ct=29.8) followed by QuickExtract and Triton. SARS-CoV-2 detection by direct rRT-PCR without RNA extraction. Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step	./cache/cord-332374-cbiw6yvb.txt	./txt/cord-332374-cbiw6yvb.txt