id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-332539-v1bfm57x	Gohl, Daryl M.	A Rapid, Cost-Effective Tailed Amplicon Method for Sequencing SARS-CoV-2	2020-05-11		.txt	text/plain	5048	246	55	Several variants of the ARTIC protocol exist in which the pooled SARS-CoV-2 amplicons from a sample are taken through a NGS library preparation protocol (using either ligation or tagmentation-based approaches) in which sample-specific barcodes are added, and are then sequenced using either short-read (Illumina) or long-read (Oxford Nanopore, PacBio) technologies. We sequenced these samples using Illumina's Nextera DNA Flex Enrichment protocol using a respiratory virus oligo panel containing probes for SARS-CoV-2, the ARTIC v3 tiled primers, and a novel tailed amplicon method designed to reduce cost and streamline the preparation of SARS-CoV-2 sequencing libraries. For the Illumina DNA Flex Enrichment protocol, SARS-CoV-2 genome coverage was more complete for samples with lower N1 and N2 Cts (ranging from ~20-30) at comparable read depths and coverage thresholds than with amplicon approaches, similar to the BEI WA isolate data ( Figure 3C , Supplemental Figure S2 -S3).	./cache/cord-332539-v1bfm57x.txt	./txt/cord-332539-v1bfm57x.txt