id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-335075-6wo2o5pp	Bangaru, Sandhya	Structural analysis of full-length SARS-CoV-2 spike protein from an advanced vaccine candidate	2020-08-06		.txt	text/plain	4715	240	51	Here, we performed cryo-EM and site-specific glycan analysis of one of the leading subunit vaccine candidates from Novavax based on a full-length spike protein formulated in polysorbate 80 (PS 80) detergent. Site-specific glycosylation of the SARS-CoV-2 prefusion spike protein produced in SF9 insect cells was analyzed using our recently described mass spectrometry proteomics-based method, involving treatment with proteases followed by sequential treatment with the endoglycosidases (Endo H and PNGase F) to introduce mass signatures in peptides with N-linked sequons (Asn-X-Thr/Ser) to assess the extent of glycosylation and the degree of glycan processing from high mannose/hybrid type to complex type (24) . In this study, we performed structural analysis of the Novavax SARS-CoV We also observed two non-spike densities within the spike trimer that corresponded with linoleic acid and polysorbate 80 detergent.	./cache/cord-335075-6wo2o5pp.txt	./txt/cord-335075-6wo2o5pp.txt