id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-336343-qbcb9qi3	Agarwal, Ajay	in-silica Analysis of SARS-CoV-2 viral strain using Reverse Vaccinology Approach: A Case Study for USA	2020-06-16		.txt	text/plain	2332	154	56	Using in-silica analysis and reverse vaccinology, two leader proteins were identified to be potential vaccine candidates for development of a multi-epitope drug. This study aims to utilize a reverse-vaccinology approach in order to identify potential vaccine candidates for COVID19 for the country USA. For MHC Class-I T-cell epitope prediction for ORF1ab leader proteins, NetMHCpan EL 4.0 method was used. The T-cell epitopes of the MHC Class-I for both the leader protein were determined using the NetMHCpan EL 4.0 prediction method of the IEDB server keeping the sequence length at 9. For MHC class-II, T-cell epitopes (HLA DRB1*04-01 allele) of the proteins were also determined using the IEDB Analysis tools. The potential T-cell epitopes, whose topology, antigenicity, allergenicity, toxicity and conservancy was analysed, for ORF1ab leader proteins MT326102 and MT326175 are depicted in the following tables. Out of the two MHC Class-I epitopes selected for leader proteins ORF1ab MT326102 and MT326175, the global energy was lowest for LSLPVLQVR.	./cache/cord-336343-qbcb9qi3.txt	./txt/cord-336343-qbcb9qi3.txt